Scoville Splice · ScovilleSplice.farm

SS-0087 — Verbal Warning

Pepper Heat Ladder Pepper · C. chinense · 3M SHU

Product Specification

Product ID
SS-0087
Product Name
Verbal Warning
Target Organism
Pepper (C. chinense)
Engineering Tier
Pepper
Series
Heat Ladder
Target SHU
3,000,000
Pun1/CS Variant
Triple
Gene Count
8
Construct Length
13,345 bp
Construct Type
T-DNA binary vector
Selection Marker
nptII (kanamycin resistance)
Promoter System
2A11
Codon Optimization
pepper
Capsaicinoid Profile
extreme multi
Burn Character
Heat Ladder: Verbal Warning

Gene Cassette Map

LB — [nptII] — [pAMT] — [Pun1/CS Triple] — [BCAT] — [KAS] — [FAR] — [PAL] — [C4H] — [4CL] — RB
GeneFunctionRole
pAMTPutative aminotransferaseVanillylamine biosynthesis
Pun1/CS (Triple)Capsaicin synthaseAcyl-vanillylamine condensation
BCATPathway enzymeCapsaicinoid pathway
KASPathway enzymeCapsaicinoid pathway
FARPathway enzymeCapsaicinoid pathway
PALPathway enzymeCapsaicinoid pathway
C4HPathway enzymeCapsaicinoid pathway
4CLPathway enzymeCapsaicinoid pathway

Flux Balance Analysis

Predicted Capsaicin
159.375 μmol/g
Predicted DHC
16.875 μmol/g
Total Flux
1.6868 μmol/h
Vanillylamine Branch
0.307
Acyl Branch
0.296
Condensation Efficiency
0.445
Growth Impact
0.571 (fraction of WT)
Feasibility
EXPLORATORY

CRISPR Guide RNAs (POX Knockout)

TargetSpacer (20-mer)PAMGC%Efficiency
CaPOX1TTAGACTTGCTGTGTATTAANGG30.0%0.914
CaPOX2ACAGGCCCTTAAGGGGGACTNGG60.0%0.712
CaPOX3TATCCGTCTATATAGGTATANGG30.0%0.657
CaPOX4CTGCAGGGCGCAACGTATGTNGG60.0%0.738
CaPOX5GCTCCGGCAGAGCCTCGGGGNGG80.0%0.866

Transformation Protocol Summary

Vector
T-DNA binary vector in Agrobacterium tumefaciens GV3101
Selection
50 mg/L kanamycin
Confirmation
PCR with construct-specific primers + HPLC capsaicinoid assay
Expected Timeline
4-6 weeks (callus to T0 plant)
Scoville Splice · Capsaicin Design Platform · Proprietary synthesized DNA construct · 2026-10-10
This product data sheet is generated as part of the Scoville Splice product package.