PepperSS-0087Heat LadderPepperProof of Concept

Verbal Warning

C. chinense ยท Heat Ladder: Verbal Warning

Scoville Heat Units

3,000,000

0
16M

18.8% of pure capsaicin

Genes

8

Construct

13,345 bp

Variant

Triple

Promoter

2A11

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

pepper

Capsaicinoid Profile

extreme multi

Gene Cassettes (8)

pAMT expression cassettevanillylamine biosynthesis
Pun1/CS (Triple) expression cassettecapsaicin synthase
BCAT expression cassettepathway enzyme
KAS expression cassettepathway enzyme
FAR expression cassettepathway enzyme
PAL expression cassettepathway enzyme
C4H expression cassettepathway enzyme
4CL expression cassettepathway enzyme

Burn Profile

Heat Ladder: Verbal Warning

Capsaicinoid blend: extreme multi

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This is one of our 10 founding pepper constructs โ€” the proof of concept for the entire Scoville Splice platform. The full package is yours: academic paper, FASTA construct, datasheet, manifest, FBA predictions, CRISPR guides, and quick-start protocol. All patented. All published on Zenodo and GenBank.