Heat Ladder

paper.html

Academic Paper

Full 13-section technical paper with abstract, pathway biochemistry, ESM2 engineering, codon optimization, FBA, CRISPR, and more

construct.fasta

DNA Construct

The complete T-DNA binary vector sequence in FASTA format — the product itself

datasheet.html

Data Sheet

One-page printable specification sheet with all key parameters

manifest.json

Technical Manifest

Machine-readable JSON spec: gene cassettes, components, promoters, selection markers

fba.json

FBA Predictions

Flux Balance Analysis yield predictions for the engineered pathway

crispr.json

CRISPR Guides

Guide RNA designs for peroxidase knockout (POX) and other interventions

README.txt

Quick-Start Protocol

Step-by-step Agrobacterium-mediated transformation guide with media compositions

original_paper.html

Published Paper

The original peer-reviewed academic paper from the Heat Ladder proof-of-concept publication